If two DNA samples are cut with different restriction enzymes (e.g., EcoRI and BamHI), what will happen when their fragments are mixed?
Text Solution
Verified by ExpertsThe correct answer is:
D
EcoRI produces 5'-AATTC-3' sticky ends. BamHI produces 5'-GATCC-3' sticky ends. Since the overhangs are not complementary, the fragments will not hybridize. For successful ligation, the same enzyme must be used to generate matching sticky ends
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